› 2ood

X-ray diffraction
2.62Å resolution

Crystal structure of guanine deaminase from Bradyrhizobium japonicum

Released:
DOI: 10.2210/pdb2ood/pdb
PDB entry 2ood coloured by chain, front view.
PDB entry 2ood coloured by chain, side view.
PDB entry 2ood coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Entry authors: Agarwal R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Guanine + H(2)O = xanthine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine deaminase Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 53.35 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89NG0 (Residues: 2-465; Coverage: 100%)
Gene name: blr3880
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GUN 1 x GUN
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6422
Unit cell:
a: 193.216Å b: 193.216Å c: 121.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.186 0.222
Expression system: Escherichia coli

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