› 2oqt

X-ray diffraction
2.41Å resolution

Structural Genomics, the crystal structure of a putative PTS IIA domain from Streptococcus pyogenes M1 GAS

Released:
DOI: 10.2210/pdb2oqt/pdb
PDB entry 2oqt coloured by chain, front view.
PDB entry 2oqt coloured by chain, side view.
PDB entry 2oqt coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Entry authors: Tan K, Wu R, Osipiuk J, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ascorbate-specific PTS system EIIA component Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 162 amino acids
Theoretical weight: 17.81 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9A1N7 (Residues: 1-161; Coverage: 100%)
Gene name: SPy_0176
Sequence domains: Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 51.224Å b: 112.781Å c: 60.401Å
α: 90° β: 99.19° γ: 90°
R-values:
R R work R free
0.21 0.208 0.254
Expression system: Escherichia coli BL21

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