› 2oqz

X-ray diffraction
1.6Å resolution

The crystal structure of sortase B from B.anthracis in complex with AAEK2

Released:
DOI: 10.2210/pdb2oqz/pdb
PDB entry 2oqz coloured by chain, front view.
PDB entry 2oqz coloured by chain, side view.
PDB entry 2oqz coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Activation of inhibitors by sortase triggers irreversible modification of the active site.
Maresso AW, Wu R, Kern JW, Zhang R, Janik D, Missiakas DM, Duban ME, Joachimiak A, Schneewind O
J Biol Chem 282 23129-39 (2007)
PMID: 17545669

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity similar to that of cathepsin L.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SrtB family sortase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 26.99 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6L8PZR0 (Residues: 35-254; Coverage: 87%)
Gene name: GBAA_4783
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
2 modified residues:
Ligand MSE 10 x MSE
Ligand CS4 1 x CS4

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 40.49Å b: 64.964Å c: 43.653Å
α: 90° β: 105.06° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Antivirulence as a new antibacterial approach for chemotherapy.
Escaich S. (2008)
16 more

2 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
1 more