› 2ory

X-ray diffraction
2.2Å resolution

Crystal structure of M37 lipase

Released:
DOI: 10.2210/pdb2ory/pdb
PDB entry 2ory coloured by chain, front view.
PDB entry 2ory coloured by chain, side view.
PDB entry 2ory coloured by chain, top view.
Source organism: Photobacterium sp. M37
Primary publication:
Structural basis for the cold adaptation of psychrophilic M37 lipase from Photobacterium lipolyticum.
Jung SK, Jeong DG, Lee MS, Lee JK, Kim HK, Ryu SE, Park BC, Kim JH, Kim SJ
Proteins 71 476-84 (2008)
PMID: 18186467

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fungal lipase-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 38.9 KDa
Source organism: Photobacterium sp. M37
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5DRN8 (Residues: 1-340; Coverage: 100%)
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 70.694Å b: 95.102Å c: 99.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.24
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Some like it cold: understanding the survival strategies of psychrophiles.
De Maayer et al. (2014)
4 more

5 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
4 more