2ost

X-ray diffraction
3.1Å resolution

The structure of a bacterial homing endonuclease : I-Ssp6803I

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-115741 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
PD(D/E)XK endonuclease domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 151 amino acids
Theoretical weight: 17.4 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q57253 (Residues: 2-150; Coverage: 99%)
Gene name: slr0915
Sequence domains: PD-(D/E)XK endonuclease
Structure domains: Trna Endonuclease; Chain: A, domain 1
Synthetic DNA 29 MER Chain: Y
Molecule details ›
Chain: Y
Length: 29 nucleotides
Theoretical weight: 8.97 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Not provided
Synthetic DNA 29 MER Chain: Z
Molecule details ›
Chain: Z
Length: 29 nucleotides
Theoretical weight: 8.86 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 143.777Å b: 143.777Å c: 319.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.278 0.328
Expression systems:
  • Escherichia coli BL21
  • Not provided