› 2osu

X-ray diffraction
2.29Å resolution

Probable glutaminase from Bacillus subtilis complexed with 6-diazo-5-oxo-L-norleucine

Released:
DOI: 10.2210/pdb2osu/pdb
PDB entry 2osu coloured by chain, front view.
PDB entry 2osu coloured by chain, side view.
PDB entry 2osu coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kim Y, Dementieva I, Vinokour E, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.06 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31465 (Residues: 1-327; Coverage: 100%)
Gene names: BSU02430, glsA, glsA1, ybgJ
Sequence domains: Glutaminase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DON 2 x DON
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 71.247Å b: 184.701Å c: 51.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.194 0.252
Expression system: Escherichia coli BL21(DE3)

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