› 2oua

X-ray diffraction
1.85Å resolution

Crystal Structure of Nocardiopsis Protease (NAPase)

Released:
DOI: 10.2210/pdb2oua/pdb
PDB entry 2oua coloured by chain, front view.
PDB entry 2oua coloured by chain, side view.
PDB entry 2oua coloured by chain, top view.
Source organism: Nocardiopsis alba
Primary publication:
Structural and mechanistic exploration of acid resistance: kinetic stability facilitates evolution of extremophilic behavior.
Kelch BA, Eagen KP, Erciyas FP, Humphris EL, Thomason AR, Mitsuiki S, Agard DA
J Mol Biol 368 870-83 (2007)
PMID: 17382344

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 19.15 KDa
Source organism: Nocardiopsis alba
UniProt:
  • Canonical: Q6K1C5 (Residues: 197-384; Coverage: 53%)
Gene name: napA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand AES 2 x AES
Ligand DIO 3 x DIO
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 61.605Å b: 61.605Å c: 184.479Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.176 0.2

Quick links

Citations

1 review citation

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes.
Bennur et al. (2014)
18 other articles

1 mention without citation

Proteolytic activation of both components of the cation stress-responsive Slt pathway in Aspergillus nidulans.
Mellado et al. (2016)