› 2ouh

X-ray diffraction
2.4Å resolution

Crystal structure of the Thrombospondin-1 N-terminal domain in complex with fractionated Heparin DP10

Released:
DOI: 10.2210/pdb2ouh/pdb
PDB entry 2ouh coloured by chain, front view.
PDB entry 2ouh coloured by chain, side view.
PDB entry 2ouh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Heparin-induced cis- and trans-dimerization modes of the thrombospondin-1 N-terminal domain.
Tan K, Duquette M, Liu JH, Shanmugasundaram K, Joachimiak A, Gallagher JT, Rigby AC, Wang JH, Lawler J
J Biol Chem 283 3932-41 (2008)
PMID: 18065761

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thrombospondin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 27.56 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P07996 (Residues: 19-257; Coverage: 21%)
Gene names: THBS1, TSP, TSP1
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 40.316Å b: 41.069Å c: 241.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.253 0.296
Expression system: Drosophila melanogaster

Quick links

Citations

5 review citations

Demystifying heparan sulfate-protein interactions.
Xu et al. (2014)
4 more

1 mention without citation

Heparin-induced cis- and trans-dimerization modes of the thrombospondin-1 N-terminal domain.
Tan et al. (2008)