› 2oui

X-ray diffraction
1.77Å resolution

D275P mutant of alcohol dehydrogenase from protozoa Entamoeba histolytica

Released:
DOI: 10.2210/pdb2oui/pdb
PDB entry 2oui coloured by chain, front view.
PDB entry 2oui coloured by chain, side view.
PDB entry 2oui coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution.
Goihberg E, Dym O, Tel-Or S, Shimon L, Frolow F, Peretz M, Burstein Y
Proteins 72 711-9 (2008)
PMID: 18260103

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 38.6 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: P35630 (Residues: 7-366; Coverage: 98%)
Gene names: ADH1, EHI_023110
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand ZN 4 x ZN
Ligand NO3 4 x NO3
Ligand CAC 4 x CAC
Ligand EDO 9 x EDO
Ligand PGE 4 x PGE
Ligand 1PE 1 x 1PE
Ligand CL 1 x CL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 145.921Å b: 144.104Å c: 80.073Å
α: 90° β: 121.44° γ: 90°
R-values:
R R work R free
0.15 0.148 0.178
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.
Hawwa et al. (2009)
7 more

1 mention without citation

A nucleobase-centered coarse-grained representation for structure prediction of RNA motifs.
Poblete et al. (2018)