› 2ouo

X-ray diffraction
1.89Å resolution

Crystal Structure of the Bromo domain 2 in human Bromodomain Containing Protein 4 (BRD4)

Released:
DOI: 10.2210/pdb2ouo/pdb
PDB entry 2ouo coloured by chain, front view.
PDB entry 2ouo coloured by chain, side view.
PDB entry 2ouo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 15.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 333-460; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 58.032Å b: 73.698Å c: 33.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli

Quick links

Citations

245 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
244 more

21 mentions without citation

Structural basis of PROTAC cooperative recognition for selective protein degradation.
Gadd et al. (2017)
20 more