› 2oux

X-ray diffraction
2.16Å resolution

Crystal structure of the soluble part of a magnesium transporter

Released:
DOI: 10.2210/pdb2oux/pdb
PDB entry 2oux coloured by chain, front view.
PDB entry 2oux coloured by chain, side view.
PDB entry 2oux coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Primary publication:
Structural studies on cytosolic domain of magnesium transporter MgtE from Enterococcus faecalis.
Ragumani S, Sauder JM, Burley SK, Swaminathan S
Proteins 78 487-91 (2010)
PMID: 19787770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium transporter MgtE Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 33.07 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q830V1 (Residues: 2-285; Coverage: 63%)
Gene names: EF_2668, mgtE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 10 x MG
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 77.215Å b: 79.662Å c: 105.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

CBS domains: Ligand binding sites and conformational variability.
Ereño-Orbea et al. (2013)
1 more

2 mentions without citation

Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE.
Tanaka et al. (2007)
1 more