› 2ovg

X-ray diffraction
1.35Å resolution

Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221

Released:
DOI: 10.2210/pdb2ovg/pdb
PDB entry 2ovg coloured by chain, front view.
PDB entry 2ovg coloured by chain, side view.
PDB entry 2ovg coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA.
Hall BM, Roberts SA, Heroux A, Cordes MH
J Mol Biol 375 802-11 (2008)
PMID: 18054042

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.36 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03040 (Residues: 1-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P3221
Unit cell:
a: 50.524Å b: 50.524Å c: 48.148Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.134 0.132 0.171
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Current View on EpCAM Structural Biology.
Gaber et al. (2020)
8 other articles

3 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
2 more