› 2ow6

X-ray diffraction
1.19Å resolution

Golgi alpha-mannosidase II complex with (1r,5s,6s,7r,8s)-1-thioniabicyclo[4.3.0]nonan-5,7,8-triol chloride

Released:
DOI: 10.2210/pdb2ow6/pdb
PDB entry 2ow6 coloured by chain, front view.
PDB entry 2ow6 coloured by chain, side view.
PDB entry 2ow6 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Binding of sulfonium-ion analogues of di-epi-swainsonine and 8-epi-lentiginosine to Drosophila Golgi alpha-mannosidase II: the role of water in inhibitor binding.
Kumar NS, Kuntz DA, Wen X, Pinto BM, Rose DR
Proteins 71 1484-96 (2008)
PMID: 18076078

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 2 x PO4
Ligand ZN 1 x ZN
Ligand NK1 1 x NK1
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 69.007Å b: 109.62Å c: 138.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.118 0.15
Expression system: Drosophila melanogaster

Quick links

Citations

1 review citation

Structure, mechanism and inhibition of Golgi α-mannosidase II.
Rose DR. (2012)
6 other articles

1 mention without citation

Structure based virtual screening of natural product molecules as glycosidase inhibitors.
Moorthy et al. (2021)