› 2owq

X-ray diffraction
2.4Å resolution

Crystal structure of vaccinia virus uracil-DNA glycosylase

Released:
DOI: 10.2210/pdb2owq/pdb
PDB entry 2owq coloured by chain, front view.
PDB entry 2owq coloured by chain, side view.
PDB entry 2owq coloured by chain, top view.
Source organism: Vaccinia virus Western Reserve
Primary publication:
Crystal structure of vaccinia virus uracil-DNA glycosylase reveals dimeric assembly.
Schormann N, Grigorian A, Samal A, Krishnan R, DeLucas L, Chattopadhyay D
BMC Struct Biol 7 45 (2007)
PMID: 17605817

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.25 KDa
Source organism: Vaccinia virus Western Reserve
Expression system: Escherichia coli
UniProt:
  • Canonical: P04303 (Residues: 1-218; Coverage: 100%)
Gene names: D4R, OPG116, UNG, VACWR109
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand IMD 3 x IMD
Ligand GOL 4 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 85.197Å b: 85.197Å c: 139.725Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.241 0.3
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.
Zharkov et al. (2010)
3 more

12 mentions without citation

Uracil-DNA glycosylases-structural and functional perspectives on an essential family of DNA repair enzymes.
Schormann et al. (2014)
11 more