2oxt

X-ray diffraction
2.9Å resolution

Crystal structure of Meaban virus nucleoside-2'-O-methyltransferase

Released:

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-106067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NUCLEOSIDE-2'-O-METHYLTRANSFERASE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 29.65 KDa
Source organism: Meaban virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0EKU1 (Residues: 2516-2780; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43
Unit cell:
a: 82.977Å b: 82.977Å c: 170.202Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.306
Expression system: Escherichia coli