Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Type II restriction enzyme EcoRI and DNA (preferred)
PDBe Complex ID:
PDB-CPX-115744 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme EcoRI
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
Length: 277 amino acids
Theoretical weight: 31.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P00642 (Residues: 1-277; Coverage: 100%)
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X6A
Spacegroup:
P321
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
