› 2oyr

X-ray diffraction
2Å resolution

Crystal Structure of UPF0341 Protein (yhiQ) from Shigella flexneri in complex with S-Adenosyl Homocysteine, Northeast Structural Genomics Target SfR275

Released:
DOI: 10.2210/pdb2oyr/pdb
PDB entry 2oyr coloured by chain, front view.
PDB entry 2oyr coloured by chain, side view.
PDB entry 2oyr coloured by chain, top view.
Source organism: Shigella flexneri 2a
Entry authors: Forouhar F, Su M, Seetharaman J, Janjua H, Fang Y, Xiao R, Baran MC, Liu J, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase J Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.31 KDa
Source organism: Shigella flexneri 2a
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7UAV7 (Residues: 1-250; Coverage: 100%)
Gene names: S4240, SF3528, rsmJ, yhiQ
Sequence domains: Putative SAM-dependent methyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 42.403Å b: 74.737Å c: 79.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.23
Expression system: Escherichia coli

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