› 2ozo

X-ray diffraction
2.6Å resolution

Autoinhibited intact human ZAP-70

Released:
DOI: 10.2210/pdb2ozo/pdb
PDB entry 2ozo coloured by chain, front view.
PDB entry 2ozo coloured by chain, side view.
PDB entry 2ozo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the inhibition of tyrosine kinase activity of ZAP-70.
Deindl S, Kadlecek TA, Brdicka T, Cao X, Weiss A, Kuriyan J
Cell 129 735-46 (2007)
PMID: 17512407

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 69.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43403 (Residues: 1-606; Coverage: 98%)
Gene names: SRK, ZAP70
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 48.297Å b: 52.929Å c: 69.331Å
α: 105.91° β: 92.94° γ: 103.72°
R-values:
R R work R free
0.26 0.22 0.29
Expression system: Spodoptera frugiperda

Quick links

Citations

42 review citations

The SYK tyrosine kinase: a crucial player in diverse biological functions.
Mócsai et al. (2010)
41 more

17 mentions without citation

ZAP-70: an essential kinase in T-cell signaling.
Wang et al. (2010)
16 more