› 2p0b

X-ray diffraction
1.74Å resolution

Crystal structure of chemically-reduced E.coli nrfB

Released:
DOI: 10.2210/pdb2p0b/pdb
PDB entry 2p0b coloured by chain, front view.
PDB entry 2p0b coloured by chain, side view.
PDB entry 2p0b coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
Clarke TA, Cole JA, Richardson DJ, Hemmings AM
Biochem J 406 19-30 (2007)
PMID: 17521287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c-type protein NrfB Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABL1 (Residues: 26-188; Coverage: 100%)
Gene names: JW4032, b4071, nrfB, yjcI
Sequence domains: Cytochrome c554 and c-prime

Ligands and Environments

1 bound ligand:
Ligand HEC 5 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 49.727Å b: 59.433Å c: 65.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.192
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The 'porin-cytochrome' model for microbe-to-mineral electron transfer.
Richardson et al. (2012)
5 more

4 mentions without citation

Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers.
Wang et al. (2019)
3 more