› 2p0f

X-ray diffraction
1.91Å resolution

ArhGAP9 PH domain in complex with Ins(1,3,5)P3

Released:
DOI: 10.2210/pdb2p0f/pdb
PDB entry 2p0f coloured by chain, front view.
PDB entry 2p0f coloured by chain, side view.
PDB entry 2p0f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Non-canonical interaction of phosphoinositides with pleckstrin homology domains of Tiam1 and ArhGAP9.
Ceccarelli DF, Blasutig IM, Goudreault M, Li Z, Ruston J, Pawson T, Sicheri F
J Biol Chem 282 13864-74 (2007)
PMID: 17339315

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GTPase-activating protein 9 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BRR9 (Residues: 321-440; Coverage: 16%)
Gene name: ARHGAP9
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 28.751Å b: 36.601Å c: 113.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.23
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Membrane recognition by phospholipid-binding domains.
Lemmon MA. (2008)
13 more

1 mention without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)