› 2p0i

X-ray diffraction
2.1Å resolution

Crystal structure of L-rhamnonate dehydratase from Gibberella zeae

Released:
DOI: 10.2210/pdb2p0i/pdb
PDB entry 2p0i coloured by chain, front view.
PDB entry 2p0i coloured by chain, side view.
PDB entry 2p0i coloured by chain, top view.
Source organism: Fusarium graminearum
Entry authors: Patskovsky Y, Toro R, Sauder JM, Dickey M, Logan C, Gheyi T, Wasserman SR, Smith D, Gerlt J, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 456 amino acids
Theoretical weight: 50.76 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VX14 (Residues: 3-448; Coverage: 98%)
Gene name: FG09883.1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 20 x GOL
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21212
Unit cell:
a: 201.404Å b: 201.135Å c: 82.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.256
Expression system: Escherichia coli BL21(DE3)

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