› 2p0j

X-ray diffraction
2.1Å resolution

Structure of restriction endonuclease BstYI bound to non-cognate DNA

Released:
DOI: 10.2210/pdb2p0j/pdb
PDB entry 2p0j coloured by chain, front view.
PDB entry 2p0j coloured by chain, side view.
PDB entry 2p0j coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
BstYI bound to noncognate DNA reveals a "hemispecific" complex: implications for DNA scanning.
Townson SA, Samuelson JC, Bao Y, Xu SY, Aggarwal AK
Structure 15 449-59 (2007)
PMID: 17437717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115749 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
BstYI Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 23.22 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84AF2 (Residues: 1-203; Coverage: 100%)
Gene name: bstYIR
Sequence domains: Restriction endonuclease BglII
Structure domains: Restriction Endonuclease
5'-D(*AP*TP*GP*AP*AP*TP*CP*CP*AP*TP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.34 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*TP*AP*TP*GP*GP*AP*TP*TP*CP*AP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 86.57Å b: 118.99Å c: 102.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 citation in other articles

Protein sliding along DNA: dynamics and structural characterization.
Givaty et al. (2009)
17 more

2 mentions without citation

Structure of the cytoplasmic domain of the flagellar secretion apparatus component FlhA from Helicobacter pylori.
Moore et al. (2010)
1 more