› 2p1j

X-ray diffraction
2.5Å resolution

Crystal structure of a polC-type DNA polymerase III exonuclease domain from Thermotoga maritima

Released:
DOI: 10.2210/pdb2p1j/pdb
PDB entry 2p1j coloured by chain, front view.
PDB entry 2p1j coloured by chain, side view.
PDB entry 2p1j coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry authors: Bonanno JB, Rutter M, Bain KT, Izuka M, Sridhar V, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III PolC-type Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.41 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZHF6 (Residues: 347-522; Coverage: 13%)
Gene names: TM_0576, polC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 55.703Å b: 124.939Å c: 143.062Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.298
Expression system: Escherichia coli BL21(DE3)

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