› 2p25

X-ray diffraction
1.7Å resolution

The crystal structure of the glyoxalase family protein from Enterococcus faecalis

Released:
DOI: 10.2210/pdb2p25/pdb
PDB entry 2p25 coloured by chain, front view.
PDB entry 2p25 coloured by chain, side view.
PDB entry 2p25 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Zhang R, Wu R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.83 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q832L2 (Residues: 1-126; Coverage: 100%)
Gene name: EF_2214
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 50.695Å b: 67.893Å c: 87.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.23
Expression system: Escherichia coli BL21(DE3)

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