› 2p2g

X-ray diffraction
2.7Å resolution

Crystal Structure of Ornithine Carbamoyltransferase from Mycobacterium Tuberculosis (Rv1656): Orthorhombic Form

Released:
DOI: 10.2210/pdb2p2g/pdb
PDB entry 2p2g coloured by chain, front view.
PDB entry 2p2g coloured by chain, side view.
PDB entry 2p2g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzyme's catalytic mechanism.
Sankaranarayanan R, Cherney MM, Cherney LT, Garen CR, Moradian F, James MN
J Mol Biol 375 1052-63 (2008)
PMID: 18062991

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine carbamoyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 307 amino acids
Theoretical weight: 33.06 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIT9 (Residues: 2-307; Coverage: 100%)
Gene names: MTCY06H11.21, Rv1656, argF
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 79.166Å b: 144.646Å c: 187.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural genomics approach to drug discovery for Mycobacterium tuberculosis.
Ioerger et al. (2009)
1 more

4 mentions without citation

The TB Structural Genomics Consortium: a decade of progress.
Chim et al. (2011)
3 more