› 2p4o

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A PUTATIVE LACTONASE OF THE SMP-30/GLUCONOLACTONASE/LRE-LIKE REGION FAMILY (NPUN_F0524) FROM NOSTOC PUNCTIFORME PCC 73102 AT 1.90 A RESOLUTION

Released:
DOI: 10.2210/pdb2p4o/pdb
PDB entry 2p4o coloured by chain, front view.
PDB entry 2p4o coloured by chain, side view.
PDB entry 2p4o coloured by chain, top view.
Source organism: Nostoc punctiforme PCC 73102
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gluconolactonase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 32.61 KDa
Source organism: Nostoc punctiforme PCC 73102
Expression system: Escherichia coli
UniProt:
  • Canonical: B2J846 (Residues: 1-305; Coverage: 100%)
Gene name: Npun_F0524
Structure domains: TolB, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P42212
Unit cell:
a: 91.442Å b: 91.442Å c: 75.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.219
Expression system: Escherichia coli

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