› 2p5k

X-ray diffraction
1Å resolution

Crystal structure of the N-terminal domain of AhrC

Released:
DOI: 10.2210/pdb2p5k/pdb
PDB entry 2p5k coloured by chain, front view.
PDB entry 2p5k coloured by chain, side view.
PDB entry 2p5k coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis.
Garnett JA, Baumberg S, Stockley PG, Phillips SE
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 914-7 (2007)
PMID: 18007039

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine repressor Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.43 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17893 (Residues: 1-64; Coverage: 43%)
Gene names: BSU24250, ahrC, argR
Sequence domains: Arginine repressor, DNA binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 25.108Å b: 32.9Å c: 66.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.16
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Structure and function of the arginine repressor-operator complex from Bacillus subtilis.
Garnett et al. (2008)
7 more

10 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
9 more