› 2p5m

X-ray diffraction
1.95Å resolution

C-terminal domain hexamer of AhrC bound with L-arginine

Released:
DOI: 10.2210/pdb2p5m/pdb
PDB entry 2p5m coloured by chain, front view.
PDB entry 2p5m coloured by chain, side view.
PDB entry 2p5m coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine.
Garnett JA, Baumberg S, Stockley PG, Phillips SE
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 918-21 (2007)
PMID: 18007040

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine repressor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 83 amino acids
Theoretical weight: 9.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17893 (Residues: 67-149; Coverage: 56%)
Gene names: BSU24250, ahrC, argR
Sequence domains: Arginine repressor, C-terminal domain
Structure domains: Gyrase A; domain 2

Ligands and Environments

1 bound ligand:
Ligand ARG 5 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P6522
Unit cell:
a: 117.014Å b: 117.014Å c: 68.927Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.182 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

Structure and function of the arginine repressor-operator complex from Bacillus subtilis.
Garnett et al. (2008)
9 more

2 mentions without citation

Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine.
Garnett et al. (2007)
1 more