› 2p5p

X-ray diffraction
2.8Å resolution

Crystal Structure Analysis of the West Nile virus envelope (E) protein domain III

Released:
DOI: 10.2210/pdb2p5p/pdb
PDB entry 2p5p coloured by chain, front view.
PDB entry 2p5p coloured by chain, side view.
PDB entry 2p5p coloured by chain, top view.
Source organism: West Nile virus
Entry authors: Gao F, Yuan F, Gao GF

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Envelope protein E Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 117 amino acids
Theoretical weight: 12.24 KDa
Source organism: West Nile virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06935 (Residues: 585-701; Coverage: 3%)
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 52.56Å b: 59.69Å c: 95.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.276
Expression system: Escherichia coli BL21

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