› 2p5u

X-ray diffraction
2.37Å resolution

Crystal structure of Thermus thermophilus HB8 UDP-glucose 4-epimerase complex with NAD

Released:
DOI: 10.2210/pdb2p5u/pdb
PDB entry 2p5u coloured by chain, front view.
PDB entry 2p5u coloured by chain, side view.
PDB entry 2p5u coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Fu Z-Q, Chen L, Ebihara A, Shinkai A, Kuramitsu S, Yokoyama S, Zhao M, Dillard B, Chrzas J, Rose JP, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG), RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178104 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 33.82 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKQ2 (Residues: 1-311; Coverage: 100%)
Gene name: TTHA0591
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 250.922Å b: 250.922Å c: 121.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.235 0.261
Expression system: Escherichia coli

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