› 2p5y

X-ray diffraction
1.92Å resolution

Crystal structure of Thermus thermophilus HB8 UDP-glucose 4-epimerase complex with NAD

Released:
DOI: 10.2210/pdb2p5y/pdb
PDB entry 2p5y coloured by chain, front view.
PDB entry 2p5y coloured by chain, side view.
PDB entry 2p5y coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Fu Z-Q, Chen L, Ebihara A, Shinkai A, Kuramitsu S, Yokoyama S, Zhu J, Swindell JT, Chrzas J, Rose JP, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG), RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 33.82 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKQ2 (Residues: 1-311; Coverage: 100%)
Gene name: TTHA0591
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P4132
Unit cell:
a: 135.667Å b: 135.667Å c: 135.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.207
Expression system: Escherichia coli

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