› 2p6z

X-ray diffraction
1.93Å resolution

Enzymatic and Structural Characterisation of Amphinase, a Novel Cytotoxic Ribonuclease from Rana pipiens Oocytes

Released:
DOI: 10.2210/pdb2p6z/pdb
PDB entry 2p6z coloured by chain, front view.
PDB entry 2p6z coloured by chain, side view.
PDB entry 2p6z coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes.
Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR
J Mol Biol 371 93-111 (2007)
PMID: 17560606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amphinase-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.24 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P85073 (Residues: 1-114; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand CIT 3 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P1
Unit cell:
a: 29.795Å b: 44.607Å c: 46.054Å
α: 116.56° β: 83.19° γ: 103.68°
R-values:
R R work R free
0.148 0.146 0.191
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity.
Rutkoski et al. (2008)
10 more

1 mention without citation

Cytostatic and cytotoxic properties of Amphinase: a novel cytotoxic ribonuclease from Rana pipiens oocytes.
Ardelt et al. (2007)