› 2p76

X-ray diffraction
2.6Å resolution

Crystal structure of a Glycerophosphodiester Phosphodiesterase from Staphylococcus aureus

Released:
DOI: 10.2210/pdb2p76/pdb
PDB entry 2p76 coloured by chain, front view.
PDB entry 2p76 coloured by chain, side view.
PDB entry 2p76 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Entry authors: Fedorov AA, Fedorov EV, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
A glycerophosphodiester + H(2)O = an alcohol + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GP-PDE domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 287 amino acids
Theoretical weight: 33.31 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JTK7 (Residues: 31-309; Coverage: 100%)
Gene name: glpQ
Sequence domains: Glycerophosphoryl diester phosphodiesterase family
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

2 bound ligands:
Ligand NA 8 x NA
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 115.164Å b: 115.164Å c: 451.335Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.265
Expression system: Escherichia coli BL21(DE3)

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