› 2pbk

X-ray diffraction
1.73Å resolution

Crystal structure of KSHV protease in complex with hexapeptide phosphonate inhibitor

Released:
DOI: 10.2210/pdb2pbk/pdb
PDB entry 2pbk coloured by chain, front view.
PDB entry 2pbk coloured by chain, side view.
PDB entry 2pbk coloured by chain, top view.
Source organism: Human gammaherpesvirus 8
Primary publication:
Substrate modulation of enzyme activity in the herpesvirus protease family.
Lazic A, Goetz DH, Nomura AM, Marnett AB, Craik CS
J Mol Biol 373 913-23 (2007)
PMID: 17870089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128741 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Assemblin Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.03 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Escherichia coli
UniProt:
  • Canonical: O36607 (Residues: 3-230; Coverage: 43%)
Sequence domains: Assemblin (Peptidase family S21)
Structure domains: Herpesvirus/Caudovirus protease domain
hexapeptide phosphonate inhibitor Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 752 Da
Source organism: Human gammaherpesvirus 8
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
1 modified residue:
Ligand TBG 2 x TBG

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 45.883Å b: 108.941Å c: 54.051Å
α: 90° β: 103.71° γ: 90°
R-values:
R R work R free
0.163 0.161 0.204
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Anti-herpesvirus agents: a patent and literature review (2003 to present).
Skoreński et al. (2014)
1 more

8 mentions without citation

Distribution and effects of amino acid changes in drug-resistant α and β herpesviruses DNA polymerase.
Topalis et al. (2016)
7 more