› 2pc5

X-ray diffraction
2.2Å resolution

Native crystal structure analysis on Arabidopsis dUTPase

Released:
DOI: 10.2210/pdb2pc5/pdb
PDB entry 2pc5 coloured by chain, front view.
PDB entry 2pc5 coloured by chain, side view.
PDB entry 2pc5 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana.
Bajaj M, Moriyama H
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 409-11 (2007)
PMID: 17565183

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 169 amino acids
Theoretical weight: 17.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9STG6 (Residues: 1-166; Coverage: 100%)
Gene names: At3g46940, DUT, DUT1, T6H20.30
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 69.903Å b: 70.86Å c: 75.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase: the role of tryptophan 93 in ligand orientation.
Inoguchi et al. (2015)
2 more

2 mentions without citation

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana.
Bajaj et al. (2007)
1 more