› 2pc6

X-ray diffraction
2.5Å resolution

Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea

Released:
DOI: 10.2210/pdb2pc6/pdb
PDB entry 2pc6 coloured by chain, front view.
PDB entry 2pc6 coloured by chain, side view.
PDB entry 2pc6 coloured by chain, top view.
Source organism: Nitrosomonas europaea ATCC 19718
Primary publication:
Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.
Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W
Protein Sci 16 1360-7 (2007)
PMID: 17586771

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetolactate synthase small subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 165 amino acids
Theoretical weight: 18.57 KDa
Source organism: Nitrosomonas europaea ATCC 19718
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82UZ2 (Residues: 1-163; Coverage: 100%)
Gene names: NE1324, ilvH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand UNL 3 x UNL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42212
Unit cell:
a: 122.123Å b: 122.123Å c: 111.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.276
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure and mechanism of inhibition of plant acetohydroxyacid synthase.
Duggleby et al. (2008)
2 more

2 mentions without citation

Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.
Petkowski et al. (2007)
1 more