› 2pc9

X-ray diffraction
2.4Å resolution

Crystal Structure Of ATP-Dependent Phosphoenolpyruvate Carboxykinase From Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2pc9/pdb
PDB entry 2pc9 coloured by chain, front view.
PDB entry 2pc9 coloured by chain, side view.
PDB entry 2pc9 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Sugahara M, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 529 amino acids
Theoretical weight: 59.39 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLL5 (Residues: 1-529; Coverage: 100%)
Gene names: TTHA0278, pckA, tthHB8IM
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand PO4 16 x PO4
Ligand ATP 4 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 109.695Å b: 128.553Å c: 178.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.25
Expression system: Escherichia coli BL21(DE3)

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