› 2pce

X-ray diffraction
2Å resolution

Crystal structure of putative mandelate racemase/muconate lactonizing enzyme from Roseovarius nubinhibens ISM

Released:
DOI: 10.2210/pdb2pce/pdb
PDB entry 2pce coloured by chain, front view.
PDB entry 2pce coloured by chain, side view.
PDB entry 2pce coloured by chain, top view.
Source organism: Roseovarius nubinhibens ISM
Entry authors: Bonanno JB, Rutter M, Bain KT, Lau C, Ozyurt S, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme, putative Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 386 amino acids
Theoretical weight: 41.1 KDa
Source organism: Roseovarius nubinhibens ISM
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3SNG0 (Residues: 1-376; Coverage: 100%)
Gene name: ISM_14080
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 8 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21212
Unit cell:
a: 168.688Å b: 174.413Å c: 108.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.25
Expression system: Escherichia coli BL21(DE3)

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