› 2pd8

X-ray diffraction
1.8Å resolution

1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid

Released:
DOI: 10.2210/pdb2pd8/pdb
PDB entry 2pd8 coloured by chain, front view.
PDB entry 2pd8 coloured by chain, side view.
PDB entry 2pd8 coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Conformational switching in the fungal light sensor Vivid.
Zoltowski BD, Schwerdtfeger C, Widom J, Loros JJ, Bilwes AM, Dunlap JC, Crane BR
Science 316 1054-7 (2007)
PMID: 17510367

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.98 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9C3Y6 (Residues: 36-184; Coverage: 80%)
Gene names: G17A4.050, vvd
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21
Unit cell:
a: 36.32Å b: 80.59Å c: 57.65Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.27 0.262 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

65 review citations

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
64 more

3 mentions without citation

Photoreceptor engineering.
Ziegler et al. (2015)
2 more