› 2pef

X-ray diffraction
1.6Å resolution

Crystal Structure of a Thermophilic Serpin, Tengpin, in the Latent State

Released:
DOI: 10.2210/pdb2pef/pdb
PDB entry 2pef coloured by chain, front view.
PDB entry 2pef coloured by chain, side view.
PDB entry 2pef coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
The N terminus of the serpin, tengpin, functions to trap the metastable native state.
Zhang Q, Buckle AM, Law RH, Pearce MC, Cabrita LD, Lloyd GJ, Irving JA, Smith AI, Ruzyla K, Rossjohn J, Bottomley SP, Whisstock JC
EMBO Rep 8 658-63 (2007)
PMID: 17557112

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serpin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 42.25 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R9P5 (Residues: 52-423; Coverage: 88%)
Gene name: TTE1560
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 44.762Å b: 44.907Å c: 159.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.253
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions.
Whisstock et al. (2010)
5 more

28 mentions without citation

Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function.
Kantyka et al. (2010)
27 more