› 2pg3

X-ray diffraction
2.4Å resolution

Crystal structure of a Queuosine biosynthesis protein queC (ECA1155) from Erwinia carotovora subsp. atroseptica SCRI1043 at 2.40 A resolution

Released:
DOI: 10.2210/pdb2pg3/pdb
PDB entry 2pg3 coloured by chain, front view.
PDB entry 2pg3 coloured by chain, side view.
PDB entry 2pg3 coloured by chain, top view.
Source organism: Pectobacterium atrosepticum SCRI1043
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
7-carboxy-7-carbaguanine + NH(3) + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-cyano-7-deazaguanine synthase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.59 KDa
Source organism: Pectobacterium atrosepticum SCRI1043
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6D820 (Residues: 1-231; Coverage: 100%)
Gene names: ECA1155, queC
Sequence domains: Queuosine biosynthesis protein QueC
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 78.154Å b: 78.154Å c: 77.755Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.223
Expression system: Escherichia coli

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