› 2ph5

X-ray diffraction
2.5Å resolution

Crystal structure of the homospermidine synthase hss from Legionella pneumophila in complex with NAD, Northeast Structural Genomics Target LgR54

Released:
DOI: 10.2210/pdb2ph5/pdb
PDB entry 2ph5 coloured by chain, front view.
PDB entry 2ph5 coloured by chain, side view.
PDB entry 2ph5 coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry authors: Forouhar F, Hussain M, Seetharaman J, Fang Y, Janjua H, Xiao R, Cunningham K, Ma L-C, Owens L, Liu J, Baran MC, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
2 putrescine = sym-homospermidine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homospermidine synthase Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 54.43 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZSM2 (Residues: 1-472; Coverage: 100%)
Gene names: hss, lpg2495
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: C2221
Unit cell:
a: 95.848Å b: 146.902Å c: 85.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.261
Expression system: Escherichia coli

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