› 2phn

X-ray diffraction
1.35Å resolution

Crystal structure of an amide bond forming F420-gamma glutamyl ligase from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2phn/pdb
PDB entry 2phn coloured by chain, front view.
PDB entry 2phn coloured by chain, side view.
PDB entry 2phn coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases.
Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A
J Mol Biol 372 456-69 (2007)
PMID: 17669425

Function and Biology Details

Reactions catalysed: 
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme F420:L-glutamate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 27.76 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28028 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2256, cofE
Sequence domains: F420-0:Gamma-glutamyl ligase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 4 x MN
Ligand ACT 4 x ACT
Ligand GDP 2 x GDP
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 98.367Å b: 98.367Å c: 94.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.16 0.19
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The many roles of glutamate in metabolism.
Walker et al. (2016)
15 other articles

6 mentions without citation

Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions.
Greening et al. (2016)
5 more