› 2pi4

X-ray diffraction
2.5Å resolution

T7RNAP complexed with a phi10 protein and initiating GTPs.

Released:
DOI: 10.2210/pdb2pi4/pdb
PDB entry 2pi4 coloured by chain, front view.
PDB entry 2pi4 coloured by chain, side view.
PDB entry 2pi4 coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase.
Kennedy WP, Momand JR, Yin YW
J Mol Biol 370 256-68 (2007)
PMID: 17512007

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115765 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 878 amino acids
Theoretical weight: 98.41 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 6-883; Coverage: 99%)
Gene name: 1
Sequence domains:
Structure domains:
5'-D(*CP*TP*TP*CP*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3' Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.73 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3' Chain: P
Molecule details ›
Chain: P
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GH3 2 x GH3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21212
Unit cell:
a: 224.45Å b: 73.791Å c: 80.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.265 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Bacteriophage protein-protein interactions.
Häuser et al. (2012)
1 more

6 mentions without citation

Computational simulation strategies for analysis of multisubunit RNA polymerases.
Wang et al. (2013)
5 more