2pic

X-ray diffraction
2.25Å resolution

E. coli lytic transglycosylase MltA-D308A in apo-2 form

Released:

Function and Biology Details

Reaction catalysed:
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-141793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-bound lytic murein transglycosylase A Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 38.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A935 (Residues: 22-365; Coverage: 100%)
Gene names: JW2784, b2813, mlt, mltA, ygdM
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 80.9Å b: 80.9Å c: 130.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.263
Expression system: Escherichia coli BL21