› 2pif

X-ray diffraction
2.3Å resolution

Crystal structure of UPF0317 protein PSPTO_5379 from Pseudomonas syringae pv. tomato. NorthEast Structural Genomics target PsR181

Released:
DOI: 10.2210/pdb2pif/pdb
PDB entry 2pif coloured by chain, front view.
PDB entry 2pif coloured by chain, side view.
PDB entry 2pif coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Entry authors: Seetharaman J, Abashidze M, Forouhar F, Wang D, Fang Y, Cunningham K, Ma L-C, Xia R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative hydro-lyase PSPTO_5379 Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 30.45 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87UC6 (Residues: 1-268; Coverage: 100%)
Gene name: PSPTO_5379
Sequence domains: D-glutamate cyclase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 66.31Å b: 85.951Å c: 93.941Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.272
Expression system: Escherichia coli

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