› 2pij

X-ray diffraction
1.7Å resolution

Structure of the Cro protein from prophage Pfl 6 in Pseudomonas fluorescens Pf-5

Released:
DOI: 10.2210/pdb2pij/pdb
PDB entry 2pij coloured by chain, front view.
PDB entry 2pij coloured by chain, side view.
PDB entry 2pij coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Primary publication:
Transitive homology-guided structural studies lead to discovery of Cro proteins with 40% sequence identity but different folds.
Roessler CG, Hall BM, Anderson WJ, Ingram WM, Roberts SA, Montfort WR, Cordes MH
Proc Natl Acad Sci U S A 105 2343-8 (2008)
PMID: 18227506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cro Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.41 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J3IU59 (Residues: 1-67; Coverage: 100%)
Gene name: PMI37_00115
Sequence domains: Cro
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand BCT 1 x BCT
Ligand DTT 1 x DTT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 40.94Å b: 40.94Å c: 123.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Proteins that switch folds.
Bryan et al. (2010)
8 more

4 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
3 more