› 2pil

X-ray diffraction
2.6Å resolution

Crystallographic Structure of Phosphorylated Pilin from Neisseria: Phosphoserine Sites Modify Type IV Pilus Surface Chemistry

Released:
DOI: 10.2210/pdb2pil/pdb
PDB entry 2pil coloured by chain, front view.
PDB entry 2pil coloured by chain, side view.
PDB entry 2pil coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Crystallographic structure reveals phosphorylated pilin from Neisseria: phosphoserine sites modify type IV pilus surface chemistry and fibre morphology.
Forest KT, Dunham SA, Koomey M, Tainer JA
Mol Microbiol 31 743-52 (1999)
PMID: 10048019

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Type IV major pilin protein PilE1 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.29 KDa
Source organism: Neisseria gonorrhoeae
UniProt:
  • Canonical: P02974 (Residues: 8-165; Coverage: 96%)
Gene name: pilE1
Sequence domains:
Structure domains: Glycoprotein, Type 4 Pilin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GLA
Carbohydrate polymer
2 bound ligands:
Ligand PT 1 x PT
Ligand HTO 1 x HTO
2 modified residues:
Ligand MEA 1 x MEA
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C222
Unit cell:
a: 127.58Å b: 121.08Å c: 26.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 not available

Quick links

Citations

17 review citations

Type IV pili and twitching motility.
Mattick JS. (2002)
16 more

9 mentions without citation

Type IV pili in Gram-positive bacteria.
Melville et al. (2013)
8 more