› 2pjr

X-ray diffraction
2.9Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115769 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
ATP-dependent DNA helicase PcrA Chains: A, F
Molecule details ›
Chains: A, F
Length: 548 amino acids
Theoretical weight: 63.37 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 1-548; Coverage: 76%)
Gene name: pcrA
Sequence domains:
Structure domains:
ATP-dependent DNA helicase PcrA Chains: B, G
Molecule details ›
Chains: B, G
Length: 95 amino acids
Theoretical weight: 10.71 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 556-650; Coverage: 13%)
Gene name: pcrA
Structure domains: Double Stranded RNA Binding Domain
DNA (5'-D(*TP*TP*TP*TP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 nucleotides
Theoretical weight: 1.48 KDa
DNA (5'-D(*GP*C)-3') Chain: H
Molecule details ›
Chain: H
Length: 2 nucleotides
Theoretical weight: 573 Da
DNA (5'-D(*AP*CP*TP*GP*C)-3') Chain: I
Molecule details ›
Chain: I
Length: 5 nucleotides
Theoretical weight: 1.48 KDa

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 85.05Å b: 62.6Å c: 141.83Å
α: 90° β: 95.84° γ: 90°
R-values:
R R work R free
0.24 0.24 0.296
Expression system: Escherichia coli

Quick links

Citations

109 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
108 more

8 mentions without citation

Structure and function of RapA: a bacterial Swi2/Snf2 protein required for RNA polymerase recycling in transcription.
Jin et al. (2011)
7 more