› 2pkf

X-ray diffraction
1.5Å resolution

Crystal structure of M tuberculosis Adenosine Kinase (apo)

Released:
DOI: 10.2210/pdb2pkf/pdb
Model geometry
Fit model/data
PDB entry 2pkf coloured by chain, front view.
PDB entry 2pkf coloured by chain, side view.
PDB entry 2pkf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme.
Reddy MCM, Palaninathan SK, Shetty ND, Owen JL, Watson MD, Sacchettini JC
J Biol Chem 282 27334-27342 (2007)
PMID: 17597075

Function and Biology Details

Reaction catalysed: 
ATP + adenosine = ADP + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 35.68 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WID5 (Residues: 1-324; Coverage: 100%)
Gene names: MTCY190.13c, Rv2202c, adoK, cbhK
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 114.272Å b: 75.184Å c: 94.73Å
α: 90° β: 121.05° γ: 90°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Adenosine kinase: exploitation for therapeutic gain.
Boison D. (2013)
3 more

5 mentions without citation

An Outline of the Latest Crystallographic Studies on Inhibitor-Enzyme Complexes for the Design and Development of New Therapeutics against Tuberculosis.
Mori et al. (2021)
4 more